Dr Gavin McStay
Pharmacy and Biomolecular Sciences
Faculty of Science
Email: G.P.McStay@ljmu.ac.uk
Telephone: 0151 904 6849
My research interests focus on the roles mitochondria play in aspects of eukaryotic life, ranging from their origin, metabolism, biogenesis and role they play in cell death pathways. These are interrogated using biochemical, molecular biology, genetic and cell biology techniques. I incorporate these interests into classes I teach to provide an understanding of practical techniques and also the current literature in the field.
I studied biochemistry at the University of Leeds, with an industrial placement at Astra Charnwood, followed by a PhD at the University of Bristol, supervised by Professor Andrew Halestrap, to characterise the molecular composition of the mitochondrial permeability transition pore, with support from MitoKor. My post-doctoral training on the mitochondrial pathway of apoptosis supervised by Dr Doug Green was at the La Jolla Institute of Allergy and Immunology in San Diego, California and St Jude Children's Research Hospital in Memphis, Tennessee. I then was an associate research scientist working in the laboratory of Professor Alexander Tzagoloff at Columbia University in New York to study biogenesis of mitochondrial cytochrome c oxidase in budding yeast, Saccharomyces cerevisiae.
I held independent positions as assistant professor at the New York Institute of Technology in New York and Staffordshire University in Stoke-on-Trent where I continued my research and taught subjects related to biochemistry, cell biology, genetics and molecular biology.
I am a strong supporter of scientific communications and outreach to disseminate scientific developments and knowledge to a broader audience with the goal of increasing the general support of the scientific process.
I also direct Biomed News, a free biomedical research literature discovery platform that uses machine learning.
Languages
English
French
German
Degrees
University of Bristol, United Kingdom, PhD, Biochemistry
University of Leeds, United Kingdom, BSc (Hons) Biochemistry with Industrial Placement
Certifications
Advance HE, United Kingdom, Fellowship
Academic appointments
Senior Lecturer, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, 2022 - present
Associate Professor, Biological Sciences, Staffordshire University, 2018 - 2022
Assistant Professor, Life Sciences, New York Institute of Technology, 2013 - 2018
Postgraduate training
Associate Research Scientist, United States, Columbia University, 2010 - 2013
Post-doctoral research, United States, St. Jude Children's Research Hospital, 2005 - 2010
Post-doctoral research, United States, La Jolla Institute For Allergy & Immunology, 2003 - 2005
Journal article
Vitry G, Finch R, Mcstay G, Behesti A, Déjean S, Larose T, Wotring V, da Silveira WA. 2022. Muscle atrophy phenotype gene expression during spaceflight is linked to a metabolic crosstalk in both the liver and the muscle in mice iScience, 25 DOI Author Url Publisher Url Public Url
Alsaffar MM, Hasan M, McStay GP, Sedky M. 2022. Digital DNA lifecycle security and privacy: An overview Briefings in Bioinformatics, 23 DOI Author Url Publisher Url Public Url
Watson SA, McStay GP. 2020. Functions of cytochrome c oxidase assembly factors International Journal of Molecular Sciences, 21 DOI Author Url Publisher Url Public Url
Barros MH, McStay GP. 2020. Modular biogenesis of mitochondrial respiratory complexes Mitochondrion, 50 :94-114 DOI Author Url Publisher Url
Franco LVR, Su CH, McStay GP, Yu GJ, Tzagoloff A. 2019. Cox2p of yeast cytochrome oxidase assembles as a stand-Alone subunit with the Cox1p and Cox3p modules Journal of Biological Chemistry, 293 :16899-16911 DOI Author Url Publisher Url
Elkholi R, Abraham-Enachescu I, Trotta AP, Rubio-Patiño C, Mohammed JN, Luna-Vargas MPA, Gelles JD, Kaminetsky JR, Serasinghe MN, Zou C, Ali S, McStay GP, Pfleger CM, Chipuk JE. 2019. MDM2 Integrates Cellular Respiration and Apoptotic Signaling through NDUFS1 and the Mitochondrial Network Molecular Cell, 74 :452-465.e7 DOI Author Url Publisher Url
Ali S, McStay GP. 2018. Regulation of mitochondrial dynamics by proteolytic processing and protein turnover Antioxidants, 7 DOI Author Url Publisher Url
McStay GP. 2017. Complex formation and turnover of mitochondrial transporters and ion channels Journal of Bioenergetics and Biomembranes, 49 :101-111 DOI Author Url Publisher Url
Su CH, McStay GP, Tzagoloff A. 2014. Assembly of the rotor component of yeast mitochondrial ATP synthase is enhanced when Atp9p is supplied by Atp9p-Cox6p complexes Journal of Biological Chemistry, 289 :31605-31616 DOI Author Url Publisher Url
McStay GP, Green DR. 2014. Identification of active caspases using affinity-based probes Cold Spring Harbor Protocols, 2014 :856-860 DOI Author Url Publisher Url
McStay GP, Green DR. 2014. Verification of a putative caspase substrate Cold Spring Harbor Protocols, 2014 :861-864 DOI Author Url Publisher Url
McStay GP, Green DR. 2014. Measuring apoptosis: Caspase inhibitors and activity assays Cold Spring Harbor Protocols, 2014 :799-806 DOI Author Url Publisher Url
McStay GP, Green DR. 2014. Preparation of cytosolic extracts and activation of caspases by cytochrome c Cold Spring Harbor Protocols, 2014 :778-782 DOI Author Url Publisher Url
McStay GP, Green DR. 2014. Ssaying caspase activity in vitro Cold Spring Harbor Protocols, 2014 :774-777 DOI Author Url Publisher Url
McStay GP, Green DR. 2014. Detection of caspase activity using antibody-based techniques Cold Spring Harbor Protocols, 2014 :783-788 DOI Author Url Publisher Url
Su CH, McStay GP, Tzagoloff A. 2014. The Cox3p assembly module of yeast cytochrome oxidase Molecular Biology of the Cell, 25 :965-976 DOI Author Url Publisher Url
Su C-H, McStay GP, Tzagoloff A. 2014. The Cox3p assembly module of yeast cytochrome oxidase MOLECULAR BIOLOGY OF THE CELL, 25 :965-976 DOI
McStay GP, Su CH, Thomas SM, Xu JT, Tzagoloff A. 2013. Characterization of assembly intermediates containing subunit 1 of yeast cytochrome oxidase Journal of Biological Chemistry, 288 :26546-26556 DOI Author Url Publisher Url
McStay GP, Su CH, Tzagoloff A. 2013. Stabilization of Cox1p intermediates by the Cox14p-Coa3p complex FEBS Letters, 587 :943-949 DOI Author Url Publisher Url
McStay GP, Su CH, Tzagoloff A. 2013. Modular assembly of yeast cytochrome oxidase Molecular Biology of the Cell, 24 :440-452 DOI Author Url Publisher Url
Bender CE, Fitzgerald P, Tait SWG, Llambi F, McStay GP, Tupper DO, Pellettieri J, Alvarado AS, Salvesen GS, Green DR. 2012. Mitochondrial pathway of apoptosis is ancestral in metazoans Proceedings of the National Academy of Sciences of the United States of America, 109 :4904-4909 DOI Author Url Publisher Url
Chipuk JE, McStay GP, Bharti A, Kuwana T, Clarke CJ, Siskind LJ, Obeid LM, Green DR. 2012. Sphingolipid metabolism cooperates with BAK and BAX to promote the mitochondrial pathway of apoptosis Cell, 148 :988-1000 DOI Author Url Publisher Url
Rak M, McStay GP, Fujikawa M, Yoshida M, Manfredi G, Tzagoloff A. 2011. Turnover of ATP synthase subunits in F
Bouchier-Hayes L, Oberst A, McStay GP, Connell S, Tait SWG, Dillon CP, Flanagan JM, Beere HM, Green DR. 2009. Characterization of Cytoplasmic Caspase-2 Activation by Induced Proximity Molecular Cell, 35 :830-840 DOI Author Url Publisher Url
McStay GP, Salvesen GS, Green DR. 2008. Overlapping cleavage motif selectivity of caspases: Implications for analysis of apoptotic pathways Cell Death and Differentiation, 15 :322-331 DOI Author Url Publisher Url
Tu S, McStay GP, Boucher LM, Mak T, Beere HM, Green DR. 2006. In situ trapping of activated initiator caspases reveals a role for caspase-2 in heat shock-induced apoptosis Nature Cell Biology, 8 :72-77 DOI Author Url Publisher Url
Spierings D, McStay G, Saleh M, Bender C, Chipuk J, Maurer U, Green DR. 2005. Connected to death: The (unexpurgated) mitochondrial pathway of apoptosis Science, 310 :66-67 DOI Author Url Publisher Url
McStay GP, Clarke SJ, Halestrap AP. 2002. Role of critical thiol groups on the matrix surface of the adenine nucleotide translocase in the mechanism of the mitochondrial permeability transition pore Biochemical Journal, 367 :541-548 DOI Author Url Publisher Url
Clarke SJ, McStay GP, Halestrap AP. 2002. Sanglifehrin A acts as a potent inhibitor of the mitochondrial permeability transition and reperfusion injury of the heart by binding to cyclophilin-D at a different site from cyclosporin A Journal of Biological Chemistry, 277 :34793-34799 DOI Author Url Publisher Url
Halestrap AP, McStay GP, Clarke SJ. 2002. The permeability transition pore complex: Another view Biochimie, 84 :153-166 DOI Author Url Publisher Url
Clarke SJ, McStay GP, Halestrap AP. 2002. Sanglifehrin A - A new inhibitor of the mitochondrial permeability transition that protects heart from reperfusion injury. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 34 :A17-A17 DOI Publisher Url
McStay GP, Clarke SJ, Halestrap AP. 2001. Identification of critical cysteine residues whose oxidative cross-linking regulates the mitochondrial permeability transition pore Biochemical Society Transactions, 29 :A78-A78 DOI Publisher Url
SCHRIRE L, CRISP S, BEAR N, MCSTAY G, KOORNHOF HJ, LEMINOR L. 1987. THE PREVALENCE OF HUMAN ISOLATES OF SALMONELLA SUBSPECIES-II IN SOUTHERN-AFRICA EPIDEMIOLOGY AND INFECTION, 98 :25-31 DOI Author Url Publisher Url
Conference publication
Vitry G, Finch R, McStay G, Behesti A, Déjean S, Larose T, Wotring V, da Silveira WA. 2022. Muscle atrophy transcriptome phenotype is linked to liver lipid metabolic processes genes expression in mice during spaceflight Proceedings of the International Astronautical Congress, IAC, 2022-September
Kaminaris A, Kobayashi S, McStay G, Liang Q. 2017. AMPK Negatively Regulates Mitophagy in the Heart FASEB JOURNAL, 31 DOI Publisher Url
McStay G, Zafar M, Tamanna F, Thomas B, Ivezic S, Chowdhury A, Noreen S. 2015. Identification of Oma1p Protease Sensitive Sites in Subunit 1 of Yeast Cytochrome Oxidase FASEB JOURNAL, 29 DOI Publisher Url
Beaupre M, Park T, McStay GP. 2015. Identification of Oma1p Protease Sensitive Sites in Subunit 1 of Yeast Cytochrome Oxidase MOLECULAR BIOLOGY OF THE CELL, 26
Chapters
Hamed M, Zaher S, McStay GP. 2020. THE ROLE OF EXECUTIONER CASPASES IN CANCER FORMATION AND MAINTENANCE Encyclopedia of Cell Biology: New Research (9 Volume Set) 4 :905-928
McStay GP. 2018. Contributions of Plasma Protein Binding and Membrane Transporters to Drug-Induced Mitochondrial Toxicity Mitochondrial Dysfunction Caused by Drugs and Environmental Toxicants 1-2 :1-14 DOI Publisher Url
McStay GP. 2016. In vitro use of peptide based substrates and inhibitors of apoptotic caspases Methods in Molecular Biology 1419 :57-67 DOI Author Url Publisher Url
Hamed M, Zaher S, McStay GP. 2016. The role of executioner caspases in cancer formation and maintenance Caspases: Roles and Significance in Cell Death and Disease :39-68
McStay GP, Green DR. 2011. Mitochondria and cell death Apoptosis: Physiology and Pathology :37-43 DOI Publisher Url
McStay G. Mitochondrial DNA Mutations in Tumourigenesis Sinha D. Mitochondria Biology of Tumourigenesis
preprint
Ali S, McStay GP. 2017. Regulation of Mitochondrial Dynamics by Proteolytic Processing and Protein Turnover MDPI AG DOI Publisher Url
Vitry G, Finch R, Mcstay G, Beheshti A, Déjean S, Larose T, Wotring V, daSilveira WA. Muscle Atrophy Phenotype Gene Expression During Spaceflight Is Linked to a Metabolic Stress Crosstalk Between the Liver and the Muscle in Mice DOI Publisher Url
Public engagement:
Museum and Gallery exhibitions/education, Adults abd families, participant, LifeArc/CamRARE ART-Translations. 2024
Participatory research, Neurodivergent children and families, Participant, British Science Association Community Buddies programme. 2023
Other Professional Activity:
MRC Future Leaders Fellowships Reviewer. 2023
BBSRC Discovery Fellowship reviewer. 2023
Cyprus Research and Innovation Foundation Small Scale Infrastructure grant review. 2023
bioRxiv pre-print screener.
External collaboration:
https://www.liverpool.ac.uk/health-and-life-sciences/lccs/, Liverpool Centre for Cardiovascular Science, Gregory Lip. 2023
Conference presentation:
Biomed News – a free machine learning based platform to help discover and share new biomedical research literature, Drug Discovery and Development, Virtual event, Poster presentation. 2023
Biomed News – a free machine learning based platform to help discover and share new biomedical research literature, Festival of Genomics 2023, London, Poster presentation. 2023
Editorial boards:
Academia Biology. 2022
Al Mustansiriyah Journal of Pharmaceutical Sciences, Editorial Board Member, https://ajps.uomustansiriyah.edu.iq/index.php/AJPS.
Frontiers in Oncology, Associate Editor for Molecular and Cellular Oncology Section, https://www.frontiersin.org/journals/oncology/sections/molecular-and-cellular-oncology.
Frontiers in Pharmacology, Review Editor for Ethnopharmacology section, https://www.frontiersin.org/journals/pharmacology/sections/ethnopharmacology.
Teaching qualification:
Advance HE fellowship. 2021
External committees:
Equality, Diversity, Inclusion panel, Biochemical Society, https://biochemistry.org/about-us/equality-diversity-and-inclusion/.
Grants committee, Biochemical Society, https://www.biochemistry.org/about-us/committees-and-governance/grants-committee/.